Gene-expression profiling of White spot syndrome virus in vivo

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Gene-expression profiling of White spot syndrome virus in vivo.

White spot syndrome virus, type species of the genus Whispovirus in the family Nimaviridae, is a large, double-stranded DNA (dsDNA) virus that infects crustaceans. The genome of the completely sequenced isolate WSSV-TH encodes 184 putative open reading frames (ORFs), the functions of which are largely unknown. To study the transcription of these ORFs, a DNA microarray was constructed, containin...

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Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate

White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...

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Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate

White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein...

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Sequence analysis of ORF94 in different White Spot Syndrome Virus (WSSV) isolates of Iran

White spot syndrome virus (WSSV) is a pathogen that causes high mortality in shrimp culture in the whole world. Sequence analysis of WSSV has shown similarity of WSSV isolates in different countries with exception of a few variable genomic loci. This study investigated the sequence variation of some Iranian WSSV isolates and previously identified isolates. Samples were collected during target ...

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Sequence analysis of ORF94 in different White Spot Syndrome Virus (WSSV) isolates of Iran

White spot syndrome virus (WSSV) is a pathogen that causes high mortality in shrimp culture in the whole world. Sequence analysis of WSSV has shown similarity of WSSV isolates in different countries with exception of a few variable genomic loci. This study investigated the sequence variation of some Iranian WSSV isolates and previously identified isolates. Samples were collected during target ...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 2005

ISSN: 0022-1317,1465-2099

DOI: 10.1099/vir.0.80895-0